Single Technologies

Single Technologies is pioneering a paradigm shift in next-generation sequencing (NGS) with its 3-D Sequencing™ platform, which moves from a flat flow cell to a three-dimensional cube structure. This architectural innovation is designed to break the throughput plateau of conventional NGS by enabling massively parallel DNA reading, while inherently combining sequencing data with spatial context. The company is developing its first product, Theta, as a 3-D Sequencer for short-read applications and is engaging with early-access partners to build novel applications in genomics, proteomics, and spatial biology. As a private, pre-revenue platform company, Single Technologies' success hinges on validating its core technology and translating its theoretical performance advantages into a commercial instrument.

3-D Sequencing™: A three-dimensional, cube-style sequencing architecture that moves beyond traditional flat flow cells. It uses a multi-layered structure to massively increase the number of parallel DNA sequencing reactions, aiming to deliver unprecedented throughput and inherently integrate sequencing data with spatial context for both in situ and solution-based applications.

Single Technologies competes in the next-generation sequencing instrument market, directly challenging the dominant flat flow cell technology of Illumina and, to a lesser extent, Thermo Fisher. Its unique spatial integration also positions it tangentially against dedicated spatial biology platforms from 10x Genomics, NanoString, and Vizgen. Its key differentiator is the promise of combining massive sequencing throughput with inherent spatial data capture.